Additional file 2: of Differential effect of angiotensin II and blood pressure on hippocampal inflammation in mice

Figure S1.. Effect of extended perfusion of subpressive Ang II on cerebral inflammation. Iba-1 (A) and GFAP (B) were examined by Western blotting in cerebral homogenates after 2 weeks systemic perfusion of Ang II 200 ng/kg/min or 0.9% saline (CTL). Densitometry values of Iba-1 and GFAP were normalized to actin, and the results are expressed relative to the control group. Representative immunoblots are shown (two-tailed Student’s t test, n = 3). CD68 mRNA (C) and GFAP mRNA (D) gene expression were analyzed by qRT-PCR in microdissected hippocampi after 2 weeks systemic perfusion of Ang II 200 ng/kg/min or 0.9% saline (CTL). In each experiment, a treated-to-control ratio was calculated (**p