GU pairs have a deleterious effect on cleavage

Copyright information:Taken from "Cleavage of dsRNAs hyper-edited by ADARs occurs at preferred editing sites"Nucleic Acids Research 2005;33(18):5954-5964.Published online 27 Oct 2005PMCID:PMC1270950.© The Author 2005. Published by Oxford University Press. All rights reserved () Cleavage assays were carried out using IIUI dsRNA molecules [5′ end-labeled on one strand (*)] and substrates where IU pairs were replaced by GU pairs (). Time points used in these assays were 0, 0.5, 1 and 2 h. () Cleavage assays were carried out using control GU dsRNA molecules [5′ end-labeled on one strand ()], and substrates where GU pairs were replaced by IU pairs. Time points used in these assays were 0, 0.5, 1 and 2 h. () Data from cleavage assays as shown in (A) and (B) were quantitated following phosphorimaging ( ≥ 4). The amount of cleaved product is given as the percentage of the total amount of dsRNA. () Cleavage assays using dsRNA substrates [5′ end-labeled on one strand ()] that contain four or five IU pairs were carried out. Time points used in these assays were 0, 0.5, 1 and 2 h. () Data from cleavage assays as shown in (D) were quantitated as described above ( ≥ 4). The amount of cleaved product is again given as the percentage of the total dsRNA. () A schematic diagram showing the position(s) of cleavage within the cleavage site sequence.