Translational control of recombinant human acetylcholinesterase accumulation in plants-6

Copyright information:Taken from "Translational control of recombinant human acetylcholinesterase accumulation in plants"http://www.biomedcentral.com/1472-6750/7/27BMC Biotechnology 2007;7():27-27.Published online 30 May 2007PMCID:PMC1913049. Nucleotide changes are shown, whereas unmodified nucleotides are represented by dashes. Above the two compared DNA sequences is the predicted amino-acid sequence of the protein. Underline: potential methylation sites. Yellow box: infrequent plant codons. Red box: potential 5'-intron splice sites. Blue box: potential downstream 3'-intron splice sites. Purple box: a potential polyadenylation positional element. Please note that the only amino-acid deviations from the human cDNA are an R2A change in the human sequence and insertion of a G residue on this position in the plant-optimized sequence. These changes were introduced to facilitate cloning into the plant expression vectors used in this study.