Additional file 3: Figure S3. of Expression of functional inhibitory neurotransmitter transporters GlyT1, GAT-1, and GAT-3 by astrocytes of inferior colliculus and hippocampus

Controls for single-cell RT-PCR. Random RNA prepared from brainstem served as positive control (p.c.). For negative control (n.c.) a patch pipette was dipped into ACSF and was placed closely to the surface of the slice without patching a cell. All targeted mRNAs were detected in p.c., whereas the n.c. was free of signals. (TIFF 1434Â kb)