Biochemical characterization of anti-LDH-5 (A) and anti-PDH (B) monoclonal antibodies by SDS-PAGE

Copyright information:Taken from "Metabolic compartmentalization in the human cortex and hippocampus: evidence for a cell- and region-specific localization of lactate dehydrogenase 5 and pyruvate dehydrogenase"http://www.biomedcentral.com/1471-2202/8/35BMC Neuroscience 2007;8():35-35.Published online 23 May 2007PMCID:PMC1899510. 1A) 1, rabbit heart; 2, rabbit muscle; 3, human LDH-1 and 4, human hippocampal extracts. 1B) 1–3, human hippocampal extracts (2, mitochondrial fraction, 3, cytosolic fraction), 4–6, human occipital cortex extracts(5, mitochondrial fraction, 6, cytosolic fraction), 7, PDH from porcine heart and 8, LDH-5 from rabbit muscle (absence of positive band at 35 kDa omitted).