Additional file 3: Figure S2. of Surfen, a proteoglycan binding agent, reduces inflammation but inhibits remyelination in murine models of Multiple Sclerosis

During EAE, surfen increases lymph node cell count, but reduces proliferation in extracted CD4 positive T cells. Superficial cervical, axillary, brachial and inguinal lymph nodes were pooled from individual mice (n shown). Total cells were counted (a) and then isolated T cells were stimulated in vivo with anti-CD3, anti CD-28 T cell expander beads for 24 h, and proliferation assessed by Oregon Green staining (b). Spleens were also homogenized (n indicates number of mice, with one spleen per mouse) and cells stained with antibodies directed against surface markers and then analyzed by flow cytometry. The percentage of CD4 positive T cells among the extracts is shown (c) along with the gating strategy (d). Data compare EAE treated with vehicle (EAE-V) or surfen (EAE-S), and is shown as mean ± SEM. Significance compares surfen with vehicle (* = P