Additional file 5: Figure S4. of Improving zebrafish embryo xenotransplantation conditions by increasing incubation temperature and establishing a proliferation index with ZFtool

Cell proliferation inside the zebrafish embryos at 34 °C and 34 °C with 5-FU (A) Zebrafish embryo incubation at 34 °C analyzed with ZFtool yielding a proliferation index of 0.4748. (B) Zebrafish embryo incubation at 34 °C, with 5-FU analyzed with the ZFtool yielding a proliferation index of 0.5415. All images are a superposition of a fluorescence field image over a bright field image. In all panels, the left image is a 48 hpf or 0 hpi zebrafish embryo, and the right image is the same zebrafish embryo with 120 hpf or 72 hpi. Scale bar = 100 μm. (TIFF 8180 kb)