HEXIM1 interacts with double-stranded, but not single-stranded nucleic acids

Copyright information:Taken from "HEXIM1 is a promiscuous double-stranded RNA-binding protein and interacts with RNAs in addition to 7SK in cultured cells"Nucleic Acids Research 2007;35(8):2503-2512.Published online 29 Mar 2007PMCID:PMC1885667.© 2007 The Author(s) () EMSA analysis of HEXIM1 with single-stranded DNA (D1, D2) and single-stranded RNA (R1, R2). Each lane contains 5 pmol of wild-type HEXIM1. () EMSA analysis of HEXIM1 (silver stain) with double-stranded DNA (D1/D2), DNA/RNA hybrids (D1/R2, D2/R1) and double-stranded RNA (R1/R2). Each lane contains 5 pmol of wild-type HEXIM1. () EMSA analysis of wild-type and ILAA mutant HEXIM1proteins with labeled single-stranded RNA (ssRNA) and double-stranded RNA (dsRNA). Each lane contains 2 pmol of wild-type or ILAA mutant HEXIM1 proteins. The top panel is a silver-stained gel showing the region containing HEXIM1, the middle panel is an autoradiograph of the same region and the bottom panel is an autoradiograph (same exposure as the middle panel) of the bottom of the gel where free-labeled probes run.