() Nuclei are isolated from cells, and aliquots are digested with a range of DNaseI as detailed in ‘Materials and methods’ section

Copyright information:Taken from "Real-time PCR mapping of DNaseI-hypersensitive sites using a novel ligation-mediated amplification technique"Nucleic Acids Research 2007;35(8):e56-e56.Published online 27 Mar 2007PMCID:PMC1885650.© 2007 The Author(s) The DNA is extracted and run on 1% agarose gels using gel electrophoresis (right panel). With the example shown, the 40-units sample gave maximal enrichment at a housekeeping promoter using the complete protocol. () The digested DNA is blunt-ended with T4 polymerase and ligated to the LP21–25 linker as detailed in ‘Materials and methods’ section. Following amplification with the biotinylated LP25 primer, the extracted DNA template represents a library of whole-genome DHS. When samples of this library are visualised using gel electrophoresis (bottom panel), the majority of products are between 300 and 500 bp in size.