Key steps of InsTet construction

Copyright information:Taken from "Integrative elements for yielding tetracycline-dependent growth phenotypes"Nucleic Acids Research 2005;33(18):e153-e153.Published online 12 Oct 2005PMCID:PMC1253839.© The Author 2005. Published by Oxford University Press. All rights reserved Closed bars symbolize single-stranded oligonucleotides for hybridization. Closed triangles represent the MEs for Tn transposase binding. Three successive stop codons are symbolized by capital ‘S’ and are given below. The Tn derived transcriptional terminator is drawn as a hairpin. The numbers 71 and 66 represent the sites and , flanking the kanamycin resistance cassette (). Open arrows denote P (for expression) and P (as regulatable outward-facing promoter). Boxes marked as ‘’ represent -operators.