Medium alkalinization of suspension cultured cells in response to UV-B irradiation and its inhibition by suramin

Copyright information:Taken from "UV-B-induced signaling events leading to enhanced-production of catharanthine in cell suspension cultures"http://www.biomedcentral.com/1471-2229/7/61BMC Plant Biology 2007;7():61-61.Published online 7 Nov 2007PMCID:PMC2213653. ()Six-day-old cell suspension...

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Hauptverfasser: Ramani, Shilpa, Jayabaskaran Chelliah
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Sprache:eng
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Zusammenfassung:Copyright information:Taken from "UV-B-induced signaling events leading to enhanced-production of catharanthine in cell suspension cultures"http://www.biomedcentral.com/1471-2229/7/61BMC Plant Biology 2007;7():61-61.Published online 7 Nov 2007PMCID:PMC2213653. ()Six-day-old cell suspension cultures were either irradiated with UV-B or left untreated for various periods of time and the pH of the medium was measured at the times indicated after the start of irradiation. Alkalinization response (AR or Δ pH) was measured as described in materials and methods. Inset: Early medium alkalinization response to 5 min of UV-B irradiation () Inhibition by suramin of UV-B-induced medium alkalinization. Cells were pre-treated with 1 mM suramin or 1 mM heparin for 10 min prior to irradiation with different doses of UV-B, and as control, cells were irradiated with UV-B alone and the pH of the medium was measured after 10 min. The increase in medium pH (Δ pH) is indicated as the difference between the pH at time 0 and at 10 min. Bars represent the means ± SD (n = 6).
DOI:10.6084/m9.figshare.56154