(A) Representative absorbance profile for RNA separated by velocity sedimentation through a 15–60% sucrose gradient

Copyright information:Taken from "Genomic analysis of the secretion stress response in the enzyme-producing cell factory "BMC Genomics 2007;8():158-158.Published online 11 Jun 2007PMCID:PMC1894978. Fractions are numbered from the top to the bottom of the gradient. (B) RNA was extracted from each fraction and subjected to electrophoresis through a formaldehyde gel. The ribosomal RNA distribution profile (25S, 18S and 5S rRNA; indicated by arrowheads) enables the the assignment of ODpeaks, corresponding to the 40S and 60S ribosomal subunits and to intact ribosomes (80S). (C) RT-PCR analysis with primers were performed from each fraction of collected gradients from treated and non-treated cells. The full length mRNA (arrow) as well as low-molecular-weight version of (arrowhead) can be visualized.