Expression vector constructs use for zebrafish transgenesis

Copyright information:Taken from "Human-zebrafish non-coding conserved elements act to regulate transcription"Nucleic Acids Research 2005;33(17):5437-5445.Published online 22 Sep 2005PMCID:PMC1236720.© The Author 2005. Published by Oxford University Press. All rights reserved The GFP construct is comprised of the zCMLC2 promoter (blue) driving expression of green fluorescent protein (green). Cloned sequences were inserted into the gateway recombination sequence (red) 5′ to the expression cassette and assessed for their ability to modulate transcriptional activity from this promoter. Similarly, the GL2 firefly luciferase construct consisted of an expression module containing the zCMLC2 promoter and the luciferase coding sequence (gray). The control pGM:RL plasmid, which contained the renilla luciferase coding sequence (purple), was used for normalization.