Cellular uptake and intracellular distribution of the fluorescein-labeled mimic oligomers (TCACTCAACACTCAC-Flu)

Copyright information:Taken from "Hydroxyproline-based DNA mimics provide an efficient gene silencing and "Nucleic Acids Research 2006;34(8):2247-2257.Published online 2 May 2006PMCID:PMC1456331.© The Author 2006. Published by Oxford University Press. All rights reserved () Fluorescent analysis of the delivery of oligomers (1 µM) into Phoenix Eco cells performed 20 h after the treatment under various delivery conditions: in the presence, or in the absence, of LFA, 6 mM CaCl, and 100 µM chloroquine (ClQ). () Confocal microscopy images of the pHypNA oligomer uptake after 20 h incubated with unfixed Phoenix Eco cells in the presence of: LFA (1); LFA/6mM Ca (2); CT-ODN/LFA (3) and LFA/100 µM ClQ (4).