S and AS transcripts detection and Msx1 protein expression from E9

Copyright information:Taken from "Expression and regulation of the natural antisense transcript during development"Nucleic Acids Research 2005;33(16):5208-5218.Published online 12 Sep 2005PMCID:PMC1214550.© The Author 2005. Published by Oxford University Press. All rights reserved5 to E14.5 in mouse anterior limb bud. Both transcripts of were detected by whole mount hybridization in wild-type mouse embryos. Msx1 protein expression was detected by the β-galactosidase activity in heterozygote mouse embryos. The AS transcript was detected in limb bud at E10.5 and E11.5 but not at E9.5 (, and ). Its detections territories edged the S transcript and protein detection territories (, , , , and ). At E14.5, AS transcript and protein show complementary expression pattern ( and ). The bar scale represent 200 µm.