Influence of different 3′ and 5′ overhangs on the subcellular localization of short dsRNAs

Copyright information:Taken from " fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5"Nucleic Acids Research 2006;34(5):1369-1380.Published online 6 Mar 2006PMCID:PMC1390680.© The Author 2006. Published by Oxford University Press. All rights reserved () Sequences and their annealing pattern of the different shifted siGL2 variants were illustrated together with their used abbreviations. For the comparison of the 3′ and the 5′ overhangs two labelled strands were used to keep the dye close to the double-stranded stem. () Quality control of the shifted GL2 short RNAs for non-annealed single strands as described in Materials and Methods with a 4% agarose gel. () Each type of short RNA labelled with Alexa488 was microinjected into HeLa SS6 cells and analysed 10–15 min later with confocal microscopy. The graph shows the ratio of fluorescence intensity nucleus/cytoplasm.