Binding affinity analysis of TIAR and mutants

Copyright information:Taken from "Novel DNA-binding properties of the RNA-binding protein TIAR"Nucleic Acids Research 2005;33(14):4507-4518.Published online 9 Aug 2005PMCID:PMC1184220.© The Author 2005. Published by Oxford University Press. All rights reserved Proteins were in molar excess of probe (indicated in legend) and were UV crosslinked as described in Materials and Methods. Bound probe was quantitated densitometrically by phosphorimaging (shown in the right panels). Affinity curves are shown on the left and the shown in the legend was extrapolated from the curve. The -axis for DNA probes is on the left and on the right for RNA probes. The curves shown are representative of a single experiment, but the analysis was repeated at least two times with similar results.