The monoclonal antibody SM5-1 recognizes a fibronectin variant which is widely expressed in melanoma-5

Copyright information:Taken from "The monoclonal antibody SM5-1 recognizes a fibronectin variant which is widely expressed in melanoma"Bmc Cancer [Electronic Resource] 2006;6():8-8.Published online 11 Jan 2006PMCID:PMC1351261.Copyright © 2006 Trefzer et al; licensee BioMed Central Ltd.by the universal M13 reverse primer in the reverse orientation. Since the range of sequenced DNA was short (a few hundred base pairs), a sequence from near the end of this run was used to design a second primer, which primed the sequence from this region and moved another few hundred nucleotides further. From the end of this run the third primer was designed and the circle was repeated until the entire insert had been covered.