Absorption () and CD spectra () of C-1305 in the presence of increasing concentrations of DNA (calf thymus DNA, poly(dA–dT) and poly(dG–dC))

Copyright information:Taken from "Induction of unique structural changes in guanine-rich DNA regions by the triazoloacridone C-1305, a topoisomerase II inhibitor with antitumor activities"Nucleic Acids Research 2005;33(18):6034-6047.Published online 27 Oct 2005PMCID:PMC1270948.© The Author 2005. Published by Oxford University Press. All rights reserved DNA titration of the drug was performed in BPE buffer at pH 7.1. To 1 ml of drug solution of 20 µM for absorption measurement and 50 µM for CD spectra, aliquots of a concentrated DNA solution were added. Vertical arrows indicate the increase of phosphate-DNA/drug ratio, horizontal arrows correspond to bathochromic shifts.