Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy-1

Copyright information:Taken from "Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy"BMC Biotechnology 2005;5():26-26.Published online 4 Oct 2005PMCID:PMC1266054.Copyright © 2005 Trieschmann et al; licensee BioMed Central Ltd.(panel A) or presence (panel B) of 10nM PrP aggregates. Panel C: quantification of measurements shown in A and B, and of measurements (not shown) with different seed concentrations. The measurements are depicted in a Fluorescence 1 (FL1-H) vs. Side-Scatter (SSC) dot-plot. Aggregate formation (signal in region R1) was strongly enhanced by all seed concentrations tested, from 5 nM to 10nM.