Comparable levels of extracellular signal-regulated kinase (ERK)1/2 phosphorylation are observed in chondrocytes treated with epidermal growth factor (EGF) alone or in combination with tumor necrosis factor alpha (TNF-α)

Copyright information:Taken from "Tumor necrosis factor alpha and epidermal growth factor act additively to inhibit matrix gene expression by chondrocyte"Arthritis Research & Therapy 2004;7(1):R127-R138.Published online 29 Nov 2004PMCID:PMC1064891.Copyright © 2004 Klooster and Bernier., licensee BioMed Central Ltd. Confluent monolayers of chondrocytes were treated for 4 hours with TNF-α (30 ng/ml) followed by 15 min treatment or 30 min treatment with EGF (10 ng/ml). Phosphorylation of ERK1/2 was determined by immunoblot assay using phospho-specific ERK1/2 antibody and ERK1 antibody (antibody against ERK1 is cross-reactive for ERK2). Blots shown are representative of three independent experiments.