Heterologous expression of the filarial nematode gene products reveals their potential to inhibit immune function-1

Copyright information:Taken from "Heterologous expression of the filarial nematode gene products reveals their potential to inhibit immune function"BMC Biology 2005;3():8-8.Published online 23 Mar 2005PMCID:PMC555940.Copyright © 2005 Gomez-Escobar et al; licensee BioMed Central Ltd.ith anti-serum or anti-ALT-1 antibody. Cloned transfected and transfected fixed parasites were incubated with an anti-ALT-1 antibody, which binds to both ALT-1 and ALT-2, and detected with an anti-mouse-FITC secondary antibody. FACS analysis of transfected promastigotes of . Fixed promastigotes were stained, without permeabilization, with anti-ALT-1 antibody and analysed by FACS. -transfected ; -transfected ; wild-type . Positive cells are those within the gate (M1) set above the threshold defined by reactivity of transfected parasites stained with normal mouse serum . Multiplication of transfectants within macrophages. -transformed wild-type amastigotes or transfected amastigotes were added to bone marrow-derived macrophages from CBA mice at a ratio of 10 parasites per macrophage. After 24 hours at 34°C the infected macrophages were observed by microscopy.