Upper panel: Kinetic traces for four different pseudo first-order hybridization reactions on 5 μm silica beads

Copyright information:Taken from "Comparative study of sequence-dependent hybridization kinetics in solution and on microspheres"Nucleic Acids Research 2005;33(1):366-375.Published online 14 Jan 2005PMCID:PMC546151.© 2005, the authors © Both a random coil (RC H) and the partial hairpin sequences (PH A′, B′ and D′) are shown. Middle panel: an expanded view of the RC H kinetic trace from above. lower panel: rate analysis for the RC H sequence. The data were fit to a straight line using a linear regression. The slope of the line gives the pseudo first-order rate constant.