Division lineages for p53-expressing fibroblasts

Copyright information:Taken from "Cellular senescence: p53-dependent asymmetric cell kinetics"Journal of Biomedicine and Biotechnology 2001;1(1):28-37.Published online Jan 2001PMCID:PMC79675.Copyright © 2001, Hindawi Publishing Corporation Shown are examples of characteristic asymmetric cell kinetics lineages observed in time-lapse analyses of p53-inducible cells (line Ind-8; 26) grown under inducing conditions (A–D) and presenescent human and murine embryonic fibroblast cell strains grown under routine culture conditions. (E) and (F), WI-38 cells at passage 18 and 26, respectively; (G), wtMEFs at passage 6. Lineages are uneven at end because cell bodies do not indicate passage of time. “Fate uncertain” indicates that a cell either migrated from the field of view or was not observed for sufficient time to assign its division state with confidence.