Interface of the Assembly PCR Oligo Maker program

Copyright information:Taken from "Assembly PCR oligo maker: a tool for designing oligodeoxynucleotides for constructing long DNA molecules for RNA production"Nucleic Acids Research 2005;33(Web Server issue):W521-W525.Published online 27 Jun 2005PMCID:PMC1160141.© The Author 2005. Published by Oxford University Press. All rights reserved The user enters the target sequence in the top window, presses the ‘Determine Oligos’ button and the output appears in the boxes below. The oligodeoxynucleotide sequences for the first PCR step are found in the ‘Assembly Oligo’ box, and the two oligodeoxynucleotide sequences for the second PCR step are found in the two ‘Flanking Primers’ boxes. User controlled settings and information about the program are accessible under the File and Help menus.