Experimental verification of the candidates predicted by ProMiR

Copyright information:Taken from "Human microRNA prediction through a probabilistic co-learning model of sequence and structure"Nucleic Acids Research 2005;33(11):3570-3581.Published online 24 Jun 2005PMCID:PMC1159118.© The Author 2005. Published by Oxford University Press. All rights reserved () Comparison of the expected PCR results for the candidates in control and Drosha knockdown HeLa cells () The left gel image shows the PCR intensity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for each cDNA. The first lane is the PCR result following silencing (si) RNA treatment for luciferase in HeLa cells for 3 days as the control and the second lane is the PCR result following siRNA for luciferase in HeLa cells for 3 days. () NC16-1 and 2, and NC19-5 and 6 are on a transcript that contains two pre-miRNAs, respectively.