Sub-cellular distribution and protein analysis of EID3

Copyright information:Taken from "EID3 is a novel EID family member and an inhibitor of CBP-dependent co-activation"Nucleic Acids Research 2005;33(11):3561-3569.Published online 24 Jun 2005PMCID:PMC1159117.© The Author 2005. Published by Oxford University Press. All rights reserved () Intracellular localization of EID1 and EID3. FLAG-tagged EID1 and EID3 were expressed in COS-7 cells and analysed by indirect immunofluorescence using FLAG antibody (green) in the absence or presence of LMB (5 nM for 5 h). Nuclei were stained with 7-aminoactinomycin D (7-AAD) (red). More than 50 cells were studied and the experiment was independently reproduced at least three times. () transcribed and translated [S]methionine-labelled EID1 and EID3 are shown.