HLA-B27 mediated expression of the α chain of the IL-2 receptor (CD25) as an activation marker on CD4CD28T cells

Copyright information:Taken from "Prevalence, clinical relevance and characterization of circulating cytotoxic CD4CD28T cells in ankylosing spondylitis"Arthritis Research & Therapy 2003;5(5):R292-R300.Published online 16 Jul 2003PMCID:PMC193730.Copyright © 2003 Duftner et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. Short term cell lines from HLA-B27 positive patients with ankylosing spondylitis were cross-linked to anti-CD3 directed immobilized antibodies (α CD3), incubated with HLA-B27 transfected C1R cells (C1R-B27) or untransfected cells (C1R) and controls, and exposed to antibodies directed against the NK receptors CD94 and CD158b/j (KIR2DL2/KIR2DL3/KIR2DS2), as indicated. Fresh peripheral blood mononuclear cells were incubated together with anti-CD3 and HLA-B27 transfected T2 cells (T2-B27) or untransfected cells (T2) as indicated, harvested after 36 hours, and surface stained for CD4, CD28 and CD25. Whiskers box blots show the results of the independent experiments, with 50% of cases within the boxes and 80% between the end-points of the whiskers (lines). The two-sided paired -test was used to determine statistical differences.