Localization of APE1 fused to MTS of the MnSOD

Copyright information:Taken from "Analysis of nuclear transport signals in the human apurinic/apyrimidinic endonuclease (APE1/Ref1)"Nucleic Acids Research 2005;33(10):3303-3312.Published online 7 Jun 2005PMCID:PMC1143697.© The Author 2005. Published by Oxford University Press. All rights reserved () Upper panel: a schematic diagram showing replacement of N1–20 amino acid residues of APE1 with MnSOD MTS at the N-terminus. The MTS-APE1 is fused with the FLAG peptide at the C-terminus. Lower panel: the BALB/c cells were transfected with cDNA encoding the MTS-APE1-FLAG, and then stained with monoclonal anti-FLAG antibody (M2) conjugated to FITC, and then with Mitotracker after fixation. () Intensities scanned along with the lines (1 and 2) denoted in the left panel, using LSMmeta (Carl Zeiss), are shown at the top plots, and correlation between Mitotracker (red) and FITC (green) are shown in the bottom plots. Arbitrary units are shown in both types of plots.