A recombinase system facilitates cloning of expression cassettes in the ciliate -4

Copyright information:Taken from "A recombinase system facilitates cloning of expression cassettes in the ciliate "BMC Microbiology 2007;7():12-12.Published online 1 Mar 2007PMCID:PMC1839094.The cassette allows the simple substitution of promoter, signal peptide (sp) gene of interest and terminator DNA sequences without loosing the flanking sites due to unique restriction sites. "Floxed" (flanked by ) expression modules were used that encode an expression cassette for the MSP1 C-terminus gene (top) and the blasticidin resistance (bsdR, bottom) gene, respectively. All expression cassettes are flanked by a histone promoter () and a beta tubulin terminator ().