A recombinase system facilitates cloning of expression cassettes in the ciliate -0

Copyright information:Taken from "A recombinase system facilitates cloning of expression cassettes in the ciliate "BMC Microbiology 2007;7():12-12.Published online 1 Mar 2007PMCID:PMC1839094. site (green) and the bacterial promoter (yellow) was inserted into pH4T2 by using the NotI and EcoRI and into pKOI by EcoRI restriction sites, respectively. The arrow head in the sites indicates the orientation. The gray/black parts correspond to the backbone sequences that are necessary for propagation in , and the red part of both acceptor vectors illustrate the selection cassette for selection in by paromomycin. The purple colored part of pAX illustrates the AT-rich double rDNA which is necessary for the propagation in . The blue part in pKOIX shows the AT-rich 5' and 3' integration sequences (bright blue, up/downstream and dark blue coding sequences). They are part of the endogenous DHFR-TS gene locus.(Bottom: detail of loxprom site).