() 600 nM core domain MCM was incubated with 3′-tailed substrate in the presence of the indicated concentrations of BSA or N-half

Copyright information:Taken from "Archaeal MCM has separable processivity, substrate choice and helicase domains"Nucleic Acids Research 2007;35(3):988-998.Published online 26 Jan 2007PMCID:PMC1807962.© 2007 The Author(s). () 300 nM core domain was incubated with 3′-tailed substrate in the presence of the indicated concentrations of wild-type N-half (WT) or N-half with a mutation in the DNA-binding β-hairpin motif (KR246AA). () 300 nM core domain was incubated with 5′-tailed substrate in the presence of the indicated concentrations of wild-type N-half (WT) or N-half with a mutation in the DNA-binding β-hairpin motif (KR246AA). () 300 nM core domain was incubated with a double-stranded DNA substrate in the presence of the indicated concentrations of wild-type N-half (WT) or N-half with a mutation in the DNA binding β-hairpin motif (KR246AA). () The core domain was incubated with various length substrates in the presence of the indicated concentrations of wild-type N-half (WT) or N-half with a mutation in the DNA binding β-hairpin motif (KR246AA), and the ratio of long:short fragments unwound was calculated.