() Chemical structure of the four base triplets employed in this work

Copyright information:Taken from "Four base recognition by triplex-forming oligonucleotides at physiological pH"Nucleic Acids Research 2005;33(9):3025-3032.Published online 23 May 2005PMCID:PMC1137030.© The Author 2005. Published by Oxford University Press. All rights reserved () Sequences of the oligonucleotides used in the fluorescence melting experiments. The duplexes are boxed and are labelled with fluorescein (F) at the 5′ end of the purine strand, whereas the TFOs are labelled with methyl red serinol (Q) at the 5′ end. Oligo 1 was used to estimate the melting temperature of duplex, whereas oligos 2 and 3 were used to verify the orientation of TFO binding. Duplexes 2–5 are identical to duplex 1, except that single base pair changes are introduced at different positions, opposite one of the modified third strand bases; positions 1.2, 3.4, 5.6 and 7.8 correspond to each base pair (A.T, T.A, G.C and C.G) in turn.