Effect of ABA-spermine cross-linking to ribosomes on translocation

Copyright information:Taken from "Localization of spermine binding sites in 23S rRNA by photoaffinity labeling: parsing the spermine contribution to ribosomal 50S subunit functions"Nucleic Acids Research 2005;33(9):2792-2805.Published online 16 May 2005PMCID:PMC1129027.© The Author 2005. Published by Oxford University Press. All rights reserved () Time course of spontaneous translocation. Aliquots of pre-translocation complex, i.e. poly(U)-programmed 70S ribosomes occupied at the P- and A-sites with tRNA and Ac[H]Phe-tRNA, respectively, were incubated for the indicated time intervals in buffer A containing 0.12 mM GTP in the absence of free spermine. The pre-translocation complex used was (filled diamonds) unlabeled, (filled circles) totally labeled or labeled in its (open circles) 50S subunit, (filled triangles) 30S subunit, or (crosses) 23S rRNA by 50 μM spermine. () Dependence of translocation on EFG concentration. The symbols are the same as those used in (A).