Proximity extension reaction specificity

Copyright information:Taken from "Proximity extension of circular DNA aptamers with real-time protein detection"Nucleic Acids Research 2005;33(6):e64-e64.Published online 7 Apr 2005PMCID:PMC1074748.© The Author 2005. Published by Oxford University Press. All rights reserved Proximity extension rates in the presence of thrombin (black squares) and trypsin (open circles). Concentrations between 100 pM and 100 nM were examined in triplicate. For all concentrations of trypsin, the maximal rate of extension over 40 min was not significantly different from that observed in the absence of either thrombin or trypsin.