Selection of zeocin-resistant clones from yeast cells expressing wt IN (), D116A IN () or no IN ()

Copyright information:Taken from "Chromosomal integration of LTR-flanked DNA in yeast expressing HIV-1 integrase: down regulation by RAD51"Nucleic Acids Research 2006;34(21):6215-6224.Published online 07 Nov 2006PMCID:PMC1693895.© 2006 The Author(s) Yeast cells (109) were grown from 0 to 5 h after transformation with the DNA integration substrate containing the two LTRs under non-selective conditions. Cells were plated on solid medium containing zeocin and after five days the zeocin-resistant clones were recovered. The results of three independent experiments are reported.