Atomic force microscopy of SeqA and 75 bp hemi-methylated DNA

Copyright information:Taken from "Dimeric configuration of SeqA protein bound to a pair of hemi-methylated GATC sequences"Nucleic Acids Research 2005;33(5):1524-1531.Published online 14 Mar 2005PMCID:PMC1065253.© The Author 2005. Published by Oxford University Press. All rights reserved () An aliquot of 10 μl of purified SeqA (50 μg/ml in buffer A containing 0.1 M KCl and no glycerol) was deposited on freshly cleaved mica and incubated for 30 min under ambient humidity to adsorb to the mica surface. Surface scanning of a 450 × 450 nm field was performed in height and amplitude mode. () Representative images of SeqA in computerized three dimensions (3D) (upper panel), and an enlarged 3D view (lower panel). The arrowhead points to the groove in the large lobe of SeqA. () Representative images of multimeric forms of SeqA molecules, with schematic pictures. A 10 μl of purified SeqA (200 μg/ml in buffer A containing 0.1 M KCl and no glycerol) was deposited on freshly cleaved mica and the surface was scanned as in (A). () A 75 bp DNA containing two hemi-sites with a spacing of 21 bp () was deposited on a Ni-treated mica surface, and images were recorded in the tapping mode in aqueous solution. The horizontal scale bars represent 20 nm, and the upright scale bars, height of the particles from baseline to the top.