Dose–response survival curves and IC values for IDCs and MDCs treated 20 hr with THI, EtHgCl, or TSA

Copyright information:Taken from "Uncoupling of ATP-Mediated Calcium Signaling and Dysregulated Interleukin-6 Secretion in Dendritic Cells by Nanomolar Thimerosal"Environmental Health Perspectives 2006;114(7):1083-1091.Published online 21 Mar 2006PMCID:PMC1513334.This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI ( and ) Survival curves plotting percent PI-negative cells versus medium control (as 100%) for IDCs () and MDCs (). () TdT DNA strand labeling and confocal microscopy of IDCs treated with medium (), 500 nM TSA (), or 500 nM THI () for 20 hr. Nuclei with strand breakage stain green; all nuclei are counterstained red with PI. Note the small and round apoptotic morphology of the THI-treated nuclei compared with controls. Original magnification, 40×; bars = 10 μm. () THI and EtHgCl inhibit [H]Ry binding to RyR1 high-affinity sites. Receptor binding analysis was performed as described in “Materials and Methods”; IC, IC, and IC values were determined by nonlinear curve fitting. TSA had no effect at the highest concentration tested. Data shown are the average of two independent experiments.