Signal intensity (log) of high (▲), medium (■) and low (●) mRNA abundances vs dilution

Copyright information:Taken from "A universal reference sample derived from clone vector for improved detection of differential gene expression"BMC Genomics 2006;7():109-109.Published online 5 May 2006PMCID:PMC1475850.Copyright © 2006 Khan et al; licensee BioMed Central Ltd. 1:25 dilution...

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Hauptverfasser: Rishi L Khan, Gonye, Gregory E, Gao, Guang, Schwaber, James S
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Sprache:eng
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Zusammenfassung:Copyright information:Taken from "A universal reference sample derived from clone vector for improved detection of differential gene expression"BMC Genomics 2006;7():109-109.Published online 5 May 2006PMCID:PMC1475850.Copyright © 2006 Khan et al; licensee BioMed Central Ltd. 1:25 dilution saturates high abundance RNA samples whereas 1:200 dilution results in undetectable signal for low abundance RNA. High, medium and low mRNA abundances. 1:50 dilution produces detectable but not saturated signal for the most spots.
DOI:10.6084/m9.figshare.41758