Immunohistochemical detection of cytosolic cytochrome-c in placental tissue in different stages of pregnancy (days 16, 19 and 21)

Copyright information:Taken from "Cancer during pregnancy alters the activity of rat placenta and enhances the expression of cleaved PARP, cytochrome-c and caspase 3"BMC Cancer 2006;6():168-168.Published online 26 Jun 2006PMCID:PMC1534057. Cytochrome-c was detected using a polyclonal antibody (Santa Cruz Biotechnology, diluted 1:100) followed by incubation with a biotinylated anti-goat antibody (1:500) and reaction with DAB in the presence of HO. The arrows indicate the cytoplasmic immunostaining of cytochrome-c in trophoblastic cells. The tissue section contains cytotrophoblastic cells (c), trophoblastic giant cells (gc) and vesicular glycogen cells (v). Groups: C – control rats, corresponding to images A, B and C on the 16, 19and 21day, respectively, W – tumour-bearing rats, corresponding to images D, E and F on the 16, 19and 21day, respectively, and A – rats injected with ascitic fluid, corresponding to images G, H and I on the 16, 19and 21day, respectively. Image J is a negative control. Magnification – 40×. Horizontal bar = 10 μm.