DNA amplification fingerprintings of breast cancer cell (MCF-7) and human mammary epithelial cell (MCF-10A)

Copyright information:Taken from "Evidence of a Genomic Biomarker in Normal Human Epithelial Mammary Cell Line, MCF-10A, That Is Absent in the Human Breast Cancer Cell Line, MCF-7"Journal of Biomedicine and Biotechnology 2006;2006():-.Published online Jan 2006PMCID:PMC1510942. Three μL of the DAF PCR amplification reaction mixture was loaded with 3 μL of loading buffer. Electrophoresis was continued at 100 V until the dye front was approximately 1 cm from the end of the gel. The amplification fragments were separated by polyacrylamide gel (5%) electrophoresis. DNA was visualized using a fast and sensitive silver staining procedure that detects 1 pg DNA/mm band cross-section. The polymorphic marker was found at 262 bps. These results were confirmed by three additional experiments.