The shows the immunocytochemical analysis of sub-cellular expression of MDR1

Copyright information:Taken from "Alternating current electrical stimulation enhanced chemotherapy: a novel strategy to bypass multidrug resistance in tumor cells"BMC Cancer 2006;6():72-72.Published online 17 Mar 2006PMCID:PMC1435916.Copyright © 2006 Janigro et al; licensee BioMed Central Ltd. Note that prior to stimulation, MDR1 was primarily located in proximity of the plasma membrane. In stimulated cells, however, MDR immunosignal significantly shifted towards the center of the cell, suggesting a cytoplasmic or nuclear localization. Quantification was achieved by plotting the levels of expression against cell diameter; the dotted red lines show these relationships. Western blot analysis () of sub-cellular fractions of cell types used for the experiments. Note that electrical stimulation caused a dramatic shift of membrane-bound MDR1 towards the cytosolic compartment. The numbers to the right show fold changes after electrical stimulation. Immunocytochemical analysis of MDR1 sub-cellular expression 48 hours post stimulation. Note that the effect of AC stimulation on MDR1 is reversible. Cells previously exposed to AC field demonstrating MDR1 cytosolic accumulation now replicates generating "normal" MDR1 + cells.