Autoradiogram of 2-D peptide maps showing dephosphorylation of phosphopetides generated from TnI excised from PKC phosphorylated rat cardiac myofibrils and dephosphorylated by PP1

Copyright information:Taken from "Dephosphorylation specificities of protein phosphatase for cardiac troponin I, troponin T, and sites within troponin T"International Journal of Biological Sciences 2006;2(1):1-9.Published online 1 Mar 2006PMCID:PMC1415850.© Ivyspring International Publisher. This is an open access article. Reproduction is permitted for personal and noncommerical use, provided that the article is in whole, unmodified, and properly cited. γ-P labeled TnI was excised from the gel, extracted, and exhaustively trypsin digested at 37°C for 17 h. The digested phosphopeptide supernatant was separated and spotted on TLC sheets. Electrophoresis was carried out in the first dimension and chromatography in the second dimension. The phosphopeptide spots were visualized by autoradiography. The findings were confirmed by three other experiments