MTT assay in HepG2 cells () and in the co-culture of HepG2 and THP1 cells () after an overnight incubation with various concentrations of extracts from , , and (positive control)

Copyright information:Taken from "Evaluation of Medicinal Plant Hepatotoxicity in Co-cultures of Hepatocytes and Monocytes"Evidence-based Complementary and Alternative Medicine 2006;3(1):93-98.Published online Jan 2006PMCID:PMC1375247.© The Author (2006). Published by Oxford University Press. All rights reserved The absorbance of the MTT formazan was determined at 570 nm in an ELISA reader. Cell viability was defined as the ratio (expressed as a percentage) of absorbance of treated cells to untreated cells. Values given represent the mean ± standard deviations of three independent experiments carried out in triplicates.