ACMV-derived siRNAs are phosphorylated at the 5 ² end RNA gel blot analysis of 20 µg total RNA prepared from ACMV-infected wild-type and treated (+) or not (â ) with alkaline phosphatase

Copyright information:Taken from "Molecular characterization of geminivirus-derived small RNAs in different plant species"Nucleic Acids Research 2006;34(2):462-471.Published online 18 Jan 2006PMCID:PMC1342034.© The Author 2006. Published by Oxford University Press. All rights reserved The blot was successively probed with DNA oligonucleotides corresponding to the ACMV DNA-A complementary (AC2 as) and virion (AC2 s) strand sequences in the AC2 coding region. Positions of the 21 and 24 nt RNAs are indicated.