Down-regulation of pol III activity in hypoxia is not due to a decrease in the abundance of TFIIIB, TFIIIC or pol III subunits

Copyright information:Taken from "Hypoxic stress suppresses RNA polymerase III recruitment and tRNA gene transcription in cardiomyocytes"Nucleic Acids Research 2006;34(1):286-294.Published online 10 Jan 2006PMCID:PMC1326236.© The Author 2006. Published by Oxford University Press. All rights reserved () Cardiomyocytes cultured in 20% (N) or 1% O (H) for 4, 8 or 16 h were resolved on an SDS–7.8% polyacrylamide gel and then analysed by western immunoblotting with antibodies specific to the 63, 90, 110 and 220 kDa subunits of TFIIIC, the TBP, Brf1 and Bdp1 subunits of TFIIIB, and the 53 and 155 kDa subunits of pol III, as indicated. () Whole-cell extract was prepared from cardiomyocytes cultured for 16 h in 1% (H1 and H2) or 20% (N1 and N2) O. Template (500 ng) VA1 was used for transcription assay using 15 µg of these extracts.