CR1 in HEXIM1 is required for its binding to P-TEFb and inhibition of transcription

Copyright information:Taken from "Oligomerization of HEXIM1 via 7SK snRNA and coiled-coil region directs the inhibition of P-TEFb"Nucleic Acids Research 2005;33(22):7000-7010.Published online 23 Dec 2005PMCID:PMC1322273.© The Author 2005. Published by Oxford University Press. All rights reserved () Disruption of the CR1 abrogates P-TEFb binding. f.Hex1 proteins were expressed in HeLa cells (6 µg, lanes 1–3) and immunoprecipitated with anti-FLAG agarose beads. Amounts of bound endogenous CycT1 is presented by WB (upper panel). The amounts of f.Hex1 proteins are indicated in WB below (lower panel). () f.Hex1mCR1 does not inhibit transcription. HeLa cells expressed pG6TAR (0.3 µg), Gal.CycT1 (1 µg) and f.Hex1 (2.7 µg) as depicted. Bars correspond to CAT values and the lower panel presents expression (WB) of f.Hex1 as indicated by the arrow.