Expression analysis of during embryonic development

Copyright information:Taken from "The phosphatidylserine receptor has essential functions during embryogenesis but not in apoptotic cell removal"Journal of Biology 2004;3(4):15-15.Published online 23 Aug 2004PMCID:PMC549712.Copyright © 2004 Böse et al., licensee BioMed Central Ltd. ) Schematic representation of the construction of the gene-trap mouse line used for expression analysis at different embryonic stages. Gray and bright blue boxes represent regulatory elements of the gene-trap, and β-geo, the β-galactosidase/neomycin phosphotransferase fusion protein-expression cassette [48,51]. Restriction enzyme nomenclature is as in Figure 1 Whole-mount β-galactosidase staining of heterozygous gene-trap embryos at mid-gestation. Expression of is highest in neural tissues and somites, in the branchial arches, the developing limbs, the heart, the primitive gut and the developing eye. Sectioning of E12.5 β-galactosidase-stained embryos confirms expression of in (c) the neural tube; (inset in c) neural epithelium; (d) somites; and (e) eyes. Expression in the eye is restricted to developing neural retinal and lens cells. Expression analysis of adult tissues by northern blot. Expression of in the muscle (asterisk) was detected only on long-term exposures of the filter (> 48 h). A hybridization was used to confirm equal loading of RNA samples. Scale bar, 100 μm.