Effect of StpA concentration on the digestion by EcoKI of EcoRI-linearized pBR322

Copyright information:Taken from "StpA protein from condenses supercoiled DNA in preference to linear DNA and protects it from digestion by DNase I and EcoKI"Nucleic Acids Research 2005;33(20):6540-6546.Published online 18 Nov 2005PMCID:PMC1289078.© The Author 2005. Published by Oxford University Press. All rights reserved Linear pBR322 (50 nM) was cut in high salt EcoKI buffer, pH 7.9, with varying concentrations of StpA for 20 min. The EcoKI concentration was 67 nM. pBR322 DNA forms a distinct band of 4.4 kb (L) which is cut to form a smear of lower molecular weight. Lane 12 contains a linear 1 kb DNA ladder of sizes 1, 1.5, 2, 3, 4, 5, 6, 8 and 10 kb from bottom to top.