Effect of StpA concentration on DNase I digestion of supercoiled pBRsk1

Effect of StpA concentration on DNase I digestion of supercoiled pBRsk1

Copyright information:Taken from "StpA protein from condenses supercoiled DNA in preference to linear DNA and protects it from digestion by DNase I and EcoKI"Nucleic Acids Research 2005;33(20):6540-6546.Published online 18 Nov 2005PMCID:PMC1289078.© The Author 2005. Published by Oxford Uni...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: S. A. Keatch, P. G. Leonard, J. E. Ladbury, D. T. F. Dryden
Format: Bild
Sprache:eng
Schlagworte:
Molecular Biology
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator S. A. Keatch
P. G. Leonard
J. E. Ladbury
D. T. F. Dryden
description Copyright information:Taken from "StpA protein from condenses supercoiled DNA in preference to linear DNA and protects it from digestion by DNase I and EcoKI"Nucleic Acids Research 2005;33(20):6540-6546.Published online 18 Nov 2005PMCID:PMC1289078.© The Author 2005. Published by Oxford University Press. All rights reserved Supercoiled pBRsk1 (CCC) (50 nM) was digested with 0.1 µg/ml DNase I for 1 h at 25°C in high salt EcoKI buffer, pH 7.9. The gel shows undigested DNA (CCC), with digested DNA being absent from the gel, and DNA protected from digestion present as linear (L) and nicked (OC) species. Lane 1 contains linear DNA markers of sizes 2, 3, 4, 5, 6, 8 and 10 kb from bottom to top.
doi_str_mv 10.6084/m9.figshare.37311
format Image
fullrecord <record><control><sourceid>datacite_PQ8</sourceid><recordid>TN_cdi_datacite_primary_10_6084_m9_figshare_37311</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_6084_m9_figshare_37311</sourcerecordid><originalsourceid>FETCH-datacite_primary_10_6084_m9_figshare_373113</originalsourceid><addsrcrecordid>eNqdzjsKAjEUBdA0FqIuwO5twDhhRJ3Sz_hpLNQ-PDIvY3AmCUks3L3_DQgXLly4cBgbioxPs_lk3BZcmzpeMBDPZ7kQXbYttSaVwGk4Jb8A5awimwIm4yw8sz5gJNhDZWqKn1FDvHkKypmGKvDLY7yKPutobCINvt1jYlOeV7tRhQmVSSR9MC2GuxSZfGFkW8gfRr4x-T-fBwgFRlQ</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>image</recordtype></control><display><type>image</type><title>Effect of StpA concentration on DNase I digestion of supercoiled pBRsk1</title><source>DataCite</source><creator>S. A. Keatch ; P. G. Leonard ; J. E. Ladbury ; D. T. F. Dryden</creator><creatorcontrib>S. A. Keatch ; P. G. Leonard ; J. E. Ladbury ; D. T. F. Dryden</creatorcontrib><description>Copyright information:Taken from "StpA protein from condenses supercoiled DNA in preference to linear DNA and protects it from digestion by DNase I and EcoKI"Nucleic Acids Research 2005;33(20):6540-6546.Published online 18 Nov 2005PMCID:PMC1289078.© The Author 2005. Published by Oxford University Press. All rights reserved Supercoiled pBRsk1 (CCC) (50 nM) was digested with 0.1 µg/ml DNase I for 1 h at 25°C in high salt EcoKI buffer, pH 7.9. The gel shows undigested DNA (CCC), with digested DNA being absent from the gel, and DNA protected from digestion present as linear (L) and nicked (OC) species. Lane 1 contains linear DNA markers of sizes 2, 3, 4, 5, 6, 8 and 10 kb from bottom to top.</description><identifier>DOI: 10.6084/m9.figshare.37311</identifier><language>eng</language><publisher>figshare</publisher><subject>Molecular Biology</subject><creationdate>2011</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>780,1894</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.6084/m9.figshare.37311$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>S. A. Keatch</creatorcontrib><creatorcontrib>P. G. Leonard</creatorcontrib><creatorcontrib>J. E. Ladbury</creatorcontrib><creatorcontrib>D. T. F. Dryden</creatorcontrib><title>Effect of StpA concentration on DNase I digestion of supercoiled pBRsk1</title><description>Copyright information:Taken from "StpA protein from condenses supercoiled DNA in preference to linear DNA and protects it from digestion by DNase I and EcoKI"Nucleic Acids Research 2005;33(20):6540-6546.Published online 18 Nov 2005PMCID:PMC1289078.© The Author 2005. Published by Oxford University Press. All rights reserved Supercoiled pBRsk1 (CCC) (50 nM) was digested with 0.1 µg/ml DNase I for 1 h at 25°C in high salt EcoKI buffer, pH 7.9. The gel shows undigested DNA (CCC), with digested DNA being absent from the gel, and DNA protected from digestion present as linear (L) and nicked (OC) species. Lane 1 contains linear DNA markers of sizes 2, 3, 4, 5, 6, 8 and 10 kb from bottom to top.</description><subject>Molecular Biology</subject><fulltext>true</fulltext><rsrctype>image</rsrctype><creationdate>2011</creationdate><recordtype>image</recordtype><sourceid>PQ8</sourceid><recordid>eNqdzjsKAjEUBdA0FqIuwO5twDhhRJ3Sz_hpLNQ-PDIvY3AmCUks3L3_DQgXLly4cBgbioxPs_lk3BZcmzpeMBDPZ7kQXbYttSaVwGk4Jb8A5awimwIm4yw8sz5gJNhDZWqKn1FDvHkKypmGKvDLY7yKPutobCINvt1jYlOeV7tRhQmVSSR9MC2GuxSZfGFkW8gfRr4x-T-fBwgFRlQ</recordid><startdate>20111231</startdate><enddate>20111231</enddate><creator>S. A. Keatch</creator><creator>P. G. Leonard</creator><creator>J. E. Ladbury</creator><creator>D. T. F. Dryden</creator><general>figshare</general><scope>DYCCY</scope><scope>PQ8</scope></search><sort><creationdate>20111231</creationdate><title>Effect of StpA concentration on DNase I digestion of supercoiled pBRsk1</title><author>S. A. Keatch ; P. G. Leonard ; J. E. Ladbury ; D. T. F. Dryden</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-datacite_primary_10_6084_m9_figshare_373113</frbrgroupid><rsrctype>images</rsrctype><prefilter>images</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Molecular Biology</topic><toplevel>online_resources</toplevel><creatorcontrib>S. A. Keatch</creatorcontrib><creatorcontrib>P. G. Leonard</creatorcontrib><creatorcontrib>J. E. Ladbury</creatorcontrib><creatorcontrib>D. T. F. Dryden</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>S. A. Keatch</au><au>P. G. Leonard</au><au>J. E. Ladbury</au><au>D. T. F. Dryden</au><format>book</format><genre>unknown</genre><ristype>GEN</ristype><title>Effect of StpA concentration on DNase I digestion of supercoiled pBRsk1</title><date>2011-12-31</date><risdate>2011</risdate><abstract>Copyright information:Taken from "StpA protein from condenses supercoiled DNA in preference to linear DNA and protects it from digestion by DNase I and EcoKI"Nucleic Acids Research 2005;33(20):6540-6546.Published online 18 Nov 2005PMCID:PMC1289078.© The Author 2005. Published by Oxford University Press. All rights reserved Supercoiled pBRsk1 (CCC) (50 nM) was digested with 0.1 µg/ml DNase I for 1 h at 25°C in high salt EcoKI buffer, pH 7.9. The gel shows undigested DNA (CCC), with digested DNA being absent from the gel, and DNA protected from digestion present as linear (L) and nicked (OC) species. Lane 1 contains linear DNA markers of sizes 2, 3, 4, 5, 6, 8 and 10 kb from bottom to top.</abstract><pub>figshare</pub><doi>10.6084/m9.figshare.37311</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier DOI: 10.6084/m9.figshare.37311
ispartof
issn
language eng
recordid cdi_datacite_primary_10_6084_m9_figshare_37311
source DataCite
subjects Molecular Biology
title Effect of StpA concentration on DNase I digestion of supercoiled pBRsk1
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T22%3A24%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-datacite_PQ8&rft_val_fmt=info:ofi/fmt:kev:mtx:book&rft.genre=unknown&rft.au=S.%20A.%20Keatch&rft.date=2011-12-31&rft_id=info:doi/10.6084/m9.figshare.37311&rft_dat=%3Cdatacite_PQ8%3E10_6084_m9_figshare_37311%3C/datacite_PQ8%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true